Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.16/2085
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degois.publication.firstPage187pt_PT
degois.publication.issue1pt_PT
degois.publication.locationEnglandpt_PT
degois.publication.titleBMC Cancerpt_PT
dc.relation.publisherversionhttps://bmccancer.biomedcentral.com/articles/10.1186/s12885-016-2228-ypt_PT
dc.contributor.authorMarques, O.-
dc.contributor.authorPorto, G.-
dc.contributor.authorRêma, A.-
dc.contributor.authorFaria, F.-
dc.contributor.authorCruz Paula, A.-
dc.contributor.authorGomez-Lazaro, M.-
dc.contributor.authorSilva, P.-
dc.contributor.authorMartins-Silva, B.-
dc.contributor.authorLopes, C.-
dc.date.accessioned2017-05-09T12:58:17Z-
dc.date.available2017-05-09T12:58:17Z-
dc.date.issued2016-03-05-
dc.identifier.citationBMC Cancer. (2016)16:187pt_PT
dc.identifier.issn1471-2407-
dc.identifier.urihttp://hdl.handle.net/10400.16/2085-
dc.description.abstractAbstract BACKGROUND: While the deregulation of iron homeostasis in breast epithelial cells is acknowledged, iron-related alterations in stromal inflammatory cells from the tumor microenvironment have not been explored. METHODS: Immunohistochemistry for hepcidin, ferroportin 1 (FPN1), transferrin receptor 1 (TFR1) and ferritin (FT) was performed in primary breast tissues and axillary lymph nodes in order to dissect the iron-profiles of epithelial cells, lymphocytes and macrophages. Furthermore, breast carcinoma core biopsies frozen in optimum cutting temperature (OCT) compound were subjected to imaging flow cytometry to confirm FPN1 expression in the cell types previously evaluated and determine its cellular localization. RESULTS: We confirm previous results by showing that breast cancer epithelial cells present an 'iron-utilization phenotype' with an increased expression of hepcidin and TFR1, and decreased expression of FT. On the other hand, lymphocytes and macrophages infiltrating primary tumors and from metastized lymph nodes display an 'iron-donor' phenotype, with increased expression of FPN1 and FT, concomitant with an activation profile reflected by a higher expression of TFR1 and hepcidin. A higher percentage of breast carcinomas, compared to control mastectomy samples, present iron accumulation in stromal inflammatory cells, suggesting that these cells may constitute an effective tissue iron reservoir. Additionally, not only the deregulated expression of iron-related proteins in epithelial cells, but also on lymphocytes and macrophages, are associated with clinicopathological markers of breast cancer poor prognosis, such as negative hormone receptor status and tumor size. CONCLUSIONS: The present results reinforce the importance of analyzing the tumor microenvironment in breast cancer, extending the contribution of immune cells to local iron homeostasis in the tumor microenvironment context.pt_PT
dc.language.isoengpt_PT
dc.publisherBioMed Centralpt_PT
dc.relationSFRH/BD/2011/78184pt_PT
dc.relationSFRH/BD/74307/2010pt_PT
dc.rightsopenAccesspt_PT
dc.subjectBreast cancerpt_PT
dc.subjectFerroportin 1pt_PT
dc.subjectIronpt_PT
dc.subjectStromal inflammatory cellspt_PT
dc.subjectTissue microenvironmentpt_PT
dc.subjectIronpt_PT
dc.subjectLymph Nodespt_PT
dc.subjectReceptors, Transferrinpt_PT
dc.subjectTumor Burdenpt_PT
dc.subjectHomeostasispt_PT
dc.subjectTumor Microenvironmentpt_PT
dc.titleLocal iron homeostasis in the breast ductal carcinoma microenvironmentpt_PT
dc.typearticlept_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.peerreviewedyespt_PT
degois.publication.volume16pt_PT
dc.identifier.doi10.1186/s12885-016-2228-ypt_PT
Aparece nas colecções:SHC - Artigos publicados em revistas indexadas na Medline

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