Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.16/565
Título: The "ex vivo" patterns of CD2/CD7, CD57/CD11c, CD38/CD11b, CD45RA/CD45RO, and CD11a/HLA‐DR expression identify acute/early and chronic/late NK‐cell activation states.
Autor: LIMA, M.
Data: Mar-2002
Editora: Elsevier
Resumo: Blood Cells Mol Dis. 2002 Mar-Apr;28(2):181-90. The "ex vivo" patterns of CD2/CD7, CD57/CD11c, CD38/CD11b, CD45RA/CD45RO, and CD11a/HLA-DR expression identify acute/early and chronic/late NK-cell activation states. Lima M, Almeida J, dos Anjos Teixeira M, Queirós ML, Justiça B, Orfão A. Service of Clinical Hematology, Hospital Geral de Santo António, Porto, Portugal. mmc.lima@clix.pt Abstract To define a dynamic sequence of phenotypic changes related to early and late phases of NK-cell activation, we have analyzed by four-color flow cytometry the immunophenotype of normal blood NK-cells from 12 healthy individuals and compared it with those from 15 patients with acute viral infections and 15 patients with either chronic infections or tumors. Although a great interindividual variability was found, nonstimulated CD56(+) NK-cells, present in normal blood samples, usually were CD2(-/+lo), CD7(+hi), HLA-DR(-), CD11b(+), CD38(+), CD11a(+hi), CD45RA(+hi), and CD45RO(-), the expression of CD11c and CD57 being heterogeneous and variable. Recently activated NK-cells, herein corresponding to NK-cells from patients with acute viral infections, displayed a pattern of expression of CD2/CD7 similar to that referred to above, but they typically showed higher levels of CD11a, CD38, and HLA-DR, as well as downregulation of CD11b and CD45RA, accompanied in some cases by coexpression of CD45RO; in addition, these NK-cells were CD11c(+) and CD57(-/+lo). Late-activated NK-cells, represented by NK-cells present in patients with chronic infections and tumors, converted into a CD2(+hi)/CD7(-/+lo) immunophenotype and expressed heterogeneously low levels of CD38 and CD11b; moreover, they were CD57(+) and CD11c(-/+). At this stage, most NK-cells had already reverted into their original CD45RA(+)/CD45RO(-)/HLA-DR(-) phenotype. In summary, we show that the patterns of expression of CD2/CD7, CD57/CD11c, CD38/CD11b, CD45RA/CD45RO, and CD11a/HLA-DR may help us to define the immunophenotypic profiles associated with early and late NK-cell activation phases in 'in vivo' models. PMID: 12064914 [PubMed - indexed for MEDLINE]
Peer review: yes
URI: http://hdl.handle.net/10400.16/565
ISSN: 1079-9796
Versão do Editor: http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WBV-462T8C5-G-1&_cdi=6720&_user=5838510&_pii=S1079979602905061&_origin=gateway&_coverDate=03%2F31%2F2002&_sk=999719997&view=c&wchp=dGLzVlb-zSkWb&md5=1acea18233e31ff8c963b700974b62cf&ie=/sdarticle.pdf
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