Browsing by Author "AROSA, F.A."
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- Human red blood cells have an enhancing effect on the relative expansion of CD8+ T lymphocytes in vitroPublication . PORTO, B.; FONSECA, A.M.; GODINHO, I.; AROSA, F.A.; PORTO, G.Cell Prolif. 2001 Dec;34(6):359-67. Human red blood cells have an enhancing effect on the relative expansion of CD8+ T lymphocytes in vitro. Porto B, Fonseca AM, Godinho I, Arosa FA, Porto G. Laboratory of Cytogenetics, Abel Salazar Institute for the Biomedical Sciences (ICBAS), Porto, Portugal. malheiro@icbas.up.pt Abstract The present study was designed to analyse the effect of red blood cells on T-cell proliferation and expansion. A comparative study was done in peripheral blood cell cultures stimulated with phytohemagglutinin, with or without red blood cells. The presence of red blood cells had a consistent enhancing effect on T lymphocyte proliferation, as determined by an increase in both the mitotic index and thymidine uptake. Phenotypic characterization of T cell blasts by flow cytometry revealed that, in the presence of red blood cells, expanding cells were preferentially CD8+ cells. Accordingly, proliferation of CD8+ lymphocytes from two patients with CD8+ hyperlymphocytosis was dependent on the presence of red blood cells. In contrast, proliferation of CD4+ lymphocytes from two patients with CD4+ hyperlymphocytosis was strongly inhibited by the presence of red blood cells. This is the first reported evidence that human red blood cells have an enhancing effect on the expansion of CD8+ lymphocytes in vitro. PMID: 11737000 [PubMed - indexed for MEDLINE]
- T cell numbers relate to bone involvement in Gaucher diseasePublication . LACERDA, L.; AROSA, F.A.; LACERDA, R.; CABEDA, J.; PORTO, G.; AMARAL, O.; Fortuna, A.; PINTO, R.; OLIVEIRA, P.; MCLAREN, C.E.; SA MIRANDA, C.; DE SOUSA, M.Blood Cells Mol Dis. 1999 Apr;25(2):130-8. T cell numbers relate to bone involvement in Gaucher disease. Lacerda L, Arosa FA, Lacerda R, Cabeda J, Porto G, Amaral O, Fortuna A, Pinto R, Oliveira P, McLaren CE, Sá Miranda C, de Sousa M. Department of Genetics Neurobiology, Porto University, Portugal. Abstract The major elements of bone pathology in Gaucher disease are a failure of osteoclast and osteoblast function, resulting in osteopenia and also osteonecrosis. T lymphocytes have recently been found to be involved in the regulation of osteoblast/osteoclast activity in vitro. In the present report the peripheral blood T major lymphocyte subsets were investigated in a group of genotyped type 1 Gaucher disease patients. A total of 31 patients were studied: 21 non-splenectomized (5 N370S homozygotes) and 10 splenectomized (of whom 1 was a N370S homozygote). The results show that non-splenectomized patients present a decrease in absolute numbers of peripheral blood T lymphocytes, specially the CD4+ T subset. However, when patients were analyzed with respect to the presence of bone disease, the number of CD8+ T lymphocytes was found to be statistically significantly lower in patients presenting bone involvement. Furthermore, lower numbers of CD8+ T lymphocytes were significantly correlated with higher levels of plasma tartrate resistant acid phosphatase (TRAP) activity, a putative marker of osteoclast cell activity. These in vivo findings are in agreement with the results reached in vitro by others. They provide an additional marker of disease severity in Gaucher disease. In the group of genotyped Gaucher disease patients, the majority of the N370S homozygous patients presented a clinically milder phenotype, including the absence of bone involvement, confirming earlier reports predicting that a number of these patients may remain undiagnosed. Collectively the homozygosity for the N370S mutation and normal T cell numbers may provide additional markers for the clinical heterogeneity of Gaucher disease. PMID: 10389595 [PubMed - indexed for MEDLINE