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Phenotypic profile of expanded NK cells in chronic lymphoproliferative disorders: a surrogate marker for NK-cell clonality

2015-12-15Journal article Open access
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dc.contributor.authorBárcena, P.
dc.contributor.authorJara-Acevedo, M.
dc.contributor.authorTabernero, M.
dc.contributor.authorLópez, A.
dc.contributor.authorSánchez, M.
dc.contributor.authorGarcía-Montero, A.
dc.contributor.authorMuñoz-García, N.
dc.contributor.authorVidriales, M.
dc.contributor.authorPaiva, A.
dc.contributor.authorLecrevisse, Q.
dc.contributor.authorLima, M.
dc.contributor.authorLangerak, A.
dc.contributor.authorBöttcher, S.
dc.contributor.authorvan Dongen, J.
dc.contributor.authorOrfao, A.
dc.contributor.authorAlmeida, J.
dc.date.accessioned2016-04-11T11:02:33Z
dc.date.available2016-04-11T11:02:33Z
dc.date.issued2015-12-15
dc.description.abstractCurrently, the lack of a universal and specific marker of clonality hampers the diagnosis and classification of chronic expansions of natural killer (NK) cells. Here we investigated the utility of flow cytometric detection of aberrant/altered NK-cell phenotypes as a surrogate marker for clonality, in the diagnostic work-up of chronic lymphoproliferative disorders of NK cells (CLPD-NK). For this purpose, a large panel of markers was evaluated by multiparametric flow cytometry on peripheral blood (PB) CD56low NK cells from 60 patients, including 23 subjects with predefined clonal (n = 9) and polyclonal (n = 14) CD56low NK-cell expansions, and 37 with CLPD-NK of undetermined clonality; also, PB samples from 10 healthy adults were included. Clonality was established using the human androgen receptor (HUMARA) assay. Clonal NK cells were found to show decreased expression of CD7, CD11b and CD38, and higher CD2, CD94 and HLADR levels vs. normal NK cells, together with a restricted repertoire of expression of the CD158a, CD158b and CD161 killer-associated receptors. In turn, NK cells from both clonal and polyclonal CLPD-NK showed similar/overlapping phenotypic profiles, except for high and more homogeneous expression of CD94 and HLADR, which was restricted to clonal CLPD-NK. We conclude that the CD94hi/HLADR+ phenotypic profile proved to be a useful surrogate marker for NK-cell clonality.pt_PT
dc.identifier.citationOncotarget. 2015 Dec 15;6(40):42938-51pt_PT
dc.identifier.doi10.18632/oncotarget.5480pt_PT
dc.identifier.issn1949-2553
dc.identifier.urihttp://hdl.handle.net/10400.16/1918
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherImpact Journalspt_PT
dc.relation.publisherversionhttp://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path[]=5480&pubmed-linkout=1pt_PT
dc.subjectCLPD-NKpt_PT
dc.subjectNK cellspt_PT
dc.subjectclonalitypt_PT
dc.subjectimmunophenotypept_PT
dc.subjectnatural killer cellspt_PT
dc.titlePhenotypic profile of expanded NK cells in chronic lymphoproliferative disorders: a surrogate marker for NK-cell clonalitypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceUnited States of Americapt_PT
oaire.citation.endPage42951pt_PT
oaire.citation.issue40pt_PT
oaire.citation.startPage42938pt_PT
oaire.citation.titleOncotargetpt_PT
oaire.citation.volume6pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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