Publication
Carbidopa Alters Tryptophan Metabolism in Breast Cancer and Melanoma Cells Leading to the Formation of Indole-3-Acetonitrile, a Pro-Proliferative Metabolite
| dc.contributor.author | Duarte, D. | |
| dc.contributor.author | Amaro, F. | |
| dc.contributor.author | Silva, I. | |
| dc.contributor.author | Silva, D. | |
| dc.contributor.author | Fresco, P. | |
| dc.contributor.author | Oliveira, José Carlos | |
| dc.contributor.author | REGUENGO, HENRIQUE | |
| dc.contributor.author | Gonçalves, J. | |
| dc.contributor.author | Vale, N. | |
| dc.date.accessioned | 2020-04-16T15:15:11Z | |
| dc.date.available | 2020-04-16T15:15:11Z | |
| dc.date.issued | 2019 | |
| dc.description.abstract | Carbidopa is used for the treatment of Parkinson's disease (PD) as an inhibitor of DOPA decarboxylase, and PD patients taking carbidopa have a lower incidence of various tumors, except for breast cancer and melanoma. Recently, it was shown that carbidopa inhibits tryptophan-2,3-dioxygenase (TDO) and kynureninase enzymes. In the present study, the effect of carbidopa on the viability and metabolic profile of breast cancer MCF-7 and melanoma A375 cells was investigated. Carbidopa was not effective in inhibiting MCF-7 and A375 proliferation. Liquid chromatography and mass spectrometry revealed a new compound, identified as indole-3-acetonitrile (IAN), which promoted a concentration-dependent increase in the viability of both cell lines. The results suggest that treatment with carbidopa may alter tryptophan (Trp) metabolism in breast cancer and melanoma leading to the formation of a pro-proliferative Trp metabolite, which may contribute to its failure in reducing breast cancers and melanoma incidence in PD patients taking carbidopa. | pt_PT |
| dc.description.sponsorship | This work was financed by FEDER—Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020—Operational Programme for Competitiveness and Internationalisation (POCI), Portugal 2020, and by Portuguese funds through FCT, in the framework of the project “Institute for Research and Innovation in Health Sciences” (POCI-01-0145-FEDER-007274).” NV thanks FCT for IF position, Fundação Manuel António da Mota (FMAM, Portugal) for support. The contents of this report are solely the responsibility of the authors and do not necessarily represent the official views of the FCT and FMAM | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Duarte D, Amaro F, Silva I, et al. Carbidopa Alters Tryptophan Metabolism in Breast Cancer and Melanoma Cells Leading to the Formation of Indole-3-Acetonitrile, a Pro-Proliferative Metabolite. Biomolecules. 2019;9(9):409. Published 2019 Aug 24. doi:10.3390/biom9090409 | pt_PT |
| dc.identifier.doi | 10.3390/biom9090409 | pt_PT |
| dc.identifier.issn | 2218-273X | |
| dc.identifier.uri | http://hdl.handle.net/10400.16/2355 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | MDPI | pt_PT |
| dc.relation.publisherversion | https://www.mdpi.com/2218-273X/9/9/409 | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | A375 cell line; MCF-7 cell line | pt_PT |
| dc.subject | carbidopa | pt_PT |
| dc.subject | indole-3-acetonitrile | pt_PT |
| dc.subject | tryptophan | pt_PT |
| dc.title | Carbidopa Alters Tryptophan Metabolism in Breast Cancer and Melanoma Cells Leading to the Formation of Indole-3-Acetonitrile, a Pro-Proliferative Metabolite | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.conferencePlace | Switzerland | pt_PT |
| oaire.citation.issue | 9 | pt_PT |
| oaire.citation.startPage | 409 | pt_PT |
| oaire.citation.title | Biomolecules | pt_PT |
| oaire.citation.volume | 9 | pt_PT |
| person.familyName | Oliveira | |
| person.familyName | Reguengo Luz | |
| person.givenName | José Carlos | |
| person.givenName | Henrique | |
| person.identifier | 806053 | |
| person.identifier.ciencia-id | 801F-D4FE-8694 | |
| person.identifier.ciencia-id | D315-6421-A327 | |
| person.identifier.orcid | 0000-0003-2142-6839 | |
| person.identifier.orcid | 0000-0002-0246-6790 | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isAuthorOfPublication | bd6d8008-2622-4368-8ded-4ec1c1160c82 | |
| relation.isAuthorOfPublication | dc00d9fd-3fff-4a83-a190-d0111a136116 | |
| relation.isAuthorOfPublication.latestForDiscovery | bd6d8008-2622-4368-8ded-4ec1c1160c82 |