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Serotonin after β-Adrenoreceptors’ Exposition: New Approaches for Personalized Data in Breast Cancer Cells

2021-09Journal article Open access
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dc.contributor.authorCorreia, Ana Salomé
dc.contributor.authorDuarte, Diana
dc.contributor.authorSilva, Isabel
dc.contributor.authorREGUENGO, HENRIQUE
dc.contributor.authorOliveira, José Carlos
dc.contributor.authorVale, Nuno
dc.date.accessioned2023-11-14T11:19:25Z
dc.date.available2023-11-14T11:19:25Z
dc.date.issued2021-09
dc.description.abstractSerotonin is an important monoamine in the human body, playing crucial roles, such as a neurotransmitter in the central nervous system. Previously, our group reported that β-adrenergic drugs (ICI 118,551, isoprenaline, and propranolol) influence the proliferation of breast cancer cells (MCF-7 cells) and their inherent production of adrenaline. Thus, we aimed to investigate the production of serotonin in MCF-7 cells, clarifying if there is a relationship between this production and the viability of the cells. To address this question, briefly, we treated the MCF-7 cells with ICI 118,551, isoprenaline, and propranolol, and evaluated cellular viability and serotonin production by using MTT, Sulforhodamine B (SRB) and Neutral Red (NR) assays, and HPLC-ECD analysis, respectively. Our results demonstrate that isoprenaline promotes the most pronounced endogenous synthesis of serotonin, about 3.5-fold greater than control cells. Propranolol treatment also increased the synthesis of serotonin (when compared to control). On the other hand, treatment with the drug ICI 118,551 promoted a lower endogenous synthesis of serotonin, about 1.1-fold less than what was observed in the control. Together, these results reveal that MCF-7 cells can produce serotonin, and the drugs propranolol, isoprenaline and ICI 118,551 influence this endogenous production. For the first time, after modulation of the β-adrenergic system, a pronounced cellular growth can be related to higher consumption of serotonin by the cells, resulting in decreased levels of serotonin in cell media, indicative of the importance of serotonin in the growth of MCF-7 cells.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCorreia AS, Duarte D, Silva I, Reguengo H, Oliveira JC, Vale N. Serotonin after β-Adrenoreceptors' Exposition: New Approaches for Personalized Data in Breast Cancer Cells. J Pers Med. 2021;11(10):954. doi:10.3390/jpm11100954pt_PT
dc.identifier.doi10.3390/jpm11100954pt_PT
dc.identifier.issn2075-4426
dc.identifier.urihttp://hdl.handle.net/10400.16/2884
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationDesenvolvimento de um novo modelo de combinação de fármacos para o combate do cancro
dc.relationNAD-5HT: New advance in the treatment of depression using serotonin
dc.relation.publisherversionhttps://www.mdpi.com/2075-4426/11/10/954pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectICI 118,551pt_PT
dc.subjectMCF-7 cellspt_PT
dc.subjectisoprenalinept_PT
dc.subjectpropranololpt_PT
dc.subjectserotoninpt_PT
dc.titleSerotonin after β-Adrenoreceptors’ Exposition: New Approaches for Personalized Data in Breast Cancer Cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDesenvolvimento de um novo modelo de combinação de fármacos para o combate do cancro
oaire.awardTitleNAD-5HT: New advance in the treatment of depression using serotonin
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F140734%2F2018/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F146093%2F2019/PT
oaire.citation.conferencePlaceSwitzerlandpt_PT
oaire.citation.issue10pt_PT
oaire.citation.startPage954pt_PT
oaire.citation.titleJournal of Personalized Medicinept_PT
oaire.citation.volume11pt_PT
oaire.fundingStreamPOR_NORTE
oaire.fundingStreamOE
person.familyNameSilva
person.familyNameReguengo Luz
person.familyNameOliveira
person.givenNameIsabel
person.givenNameHenrique
person.givenNameJosé Carlos
person.identifier806053
person.identifier.ciencia-idD315-6421-A327
person.identifier.ciencia-id801F-D4FE-8694
person.identifier.orcid0000-0001-7244-4792
person.identifier.orcid0000-0002-0246-6790
person.identifier.orcid0000-0003-2142-6839
person.identifier.ridK-9890-2014
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscoveryb63c909d-b3f9-4507-bd37-99e7740ec2b1
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