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Non-Transferrin-Bound Iron (NTBI) Uptake by T Lymphocytes: Evidence for the Selective Acquisition of Oligomeric Ferric Citrate Species

2013Journal article Open access
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dc.contributor.authorArezes, J.
dc.contributor.authorCosta, M.
dc.contributor.authorVieira, I.
dc.contributor.authorDias, V.
dc.contributor.authorKong, X.
dc.contributor.authorFernandes, R.
dc.contributor.authorVos, M.
dc.contributor.authorCarlsson, A.
dc.contributor.authorRikers, Y.
dc.contributor.authorPorto, G.
dc.contributor.authorRangel, M.
dc.contributor.authorHider, R.
dc.contributor.authorPinto, J.
dc.date.accessioned2014-10-14T15:09:47Z
dc.date.available2014-10-14T15:09:47Z
dc.date.issued2013
dc.description.abstractIron is an essential nutrient in several biological processes such as oxygen transport, DNA replication and erythropoiesis. Plasma iron normally circulates bound to transferrin. In iron overload disorders, however, iron concentrations exceed transferrin binding capacity and iron appears complexed with low molecular weight molecules, known as non-transferrin-bound iron (NTBI). NTBI is responsible for the toxicity associated with iron-overload pathologies but the mechanisms leading to NTBI uptake are not fully understood. Here we show for the first time that T lymphocytes are able to take up and accumulate NTBI in a manner that resembles that of hepatocytes. Moreover, we show that both hepatocytes and T lymphocytes take up the oligomeric Fe3Cit3 preferentially to other iron-citrate species, suggesting the existence of a selective NTBI carrier. These results provide a tool for the identification of the still elusive ferric-citrate cellular carrier and may also open a new pathway towards the design of more efficient iron chelators for the treatment of iron overload disorders.por
dc.description.sponsorshipThis work is funded by FEDER funds through the Operational Competitiveness Programme – COMPETE, by national funds through FCT – Fundação para a Ciência e a Tecnologia under the projects FCOMP-01-0124-FEDER-015823(PTDC/SAU-MET/​113011/2009),FCOMP-01-0124-FEDER-007046(PTDC/BIA-BCM/​66818/2006)FCOMP-01-0124-FEDER-007506(PTDC/SAU-GMG/​67868/2006)and by the INOVA Foundation. JA, IV, MC and VD are recipients of FCT fellowships (www.fct.pt). JPP is supported by Programa Ciência (sponsored by POPH-QREN (4.2), with match-funding from the European Social Fund and Portuguese funds from the MCTES). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.por
dc.identifier.citationArezes J, Costa M, Vieira I, Dias V, Kong XL, et al. (2013) Non-Transferrin-Bound Iron (NTBI) Uptake by T Lymphocytes: Evidence for the Selective Acquisition of Oligomeric Ferric Citrate Species. PLoS ONE 8(11): e79870. doi:10.1371/journal.pone.0079870por
dc.identifier.doi10.1371/journal.pone.0079870
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10400.16/1696
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherPublic Library of Sciencepor
dc.relationHFE as a multitask protein: genomic and biochemical analysis
dc.relation.publisherversionhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0079870por
dc.titleNon-Transferrin-Bound Iron (NTBI) Uptake by T Lymphocytes: Evidence for the Selective Acquisition of Oligomeric Ferric Citrate Speciespor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHFE as a multitask protein: genomic and biochemical analysis
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FSAU-MET%2F113011%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FBIA-BCM%2F66818%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FSAU-GMG%2F67868%2F2006/PT
oaire.citation.conferencePlaceUnited States of Americapor
oaire.citation.titlePloS onepor
oaire.citation.volume8(11)por
oaire.fundingStream5876-PPCDTI
oaire.fundingStream5876-PPCDTI
oaire.fundingStream5876-PPCDTI
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typearticlepor
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relation.isProjectOfPublicationc30247a9-16e5-46db-bd2e-dcc9e446ab4e
relation.isProjectOfPublication3a81d7a1-2f00-4d10-b4d7-ae18db766bb3
relation.isProjectOfPublication.latestForDiscovery1c01be31-0e6d-4998-9eb4-dcf2564ffe8e

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