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Linkage of typically cytosolic peroxidases to erythrocyte membrane - A possible mechanism of protection in Hereditary Spherocytosis

2020Journal article Open access
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dc.contributor.authorRocha, Susana
dc.contributor.authorRocha-Pereira, Petronila
dc.contributor.authorCleto, Maria Esmeralda Da Silva
dc.contributor.authorFerreira, Fátima
dc.contributor.authorBelo, Luís
dc.contributor.authorSantos-Silva, Alice
dc.date.accessioned2021-07-06T17:55:50Z
dc.date.available2021-07-06T17:55:50Z
dc.date.issued2020
dc.description.abstractHereditary Spherocytosis (HS) is a non-immune hemolytic anemia associated to oxidative stress (OS), namely to the linkage of cytosolic antioxidant enzymes to the erythrocyte membrane. Our aims were to evaluate erythrocyte OS changes and the membrane linkage of peroxiredoxin 2 (Prx2), glutathione peroxidase (GPx) and catalase (CAT) in unsplenectomized (unspl) and splenectomized (spl) HS patients and to search for associations with clinical severity (in unspl HS patients). We studied 114 HS patients (74 unspl and 40 spl) and 30 healthy individuals and we evaluated membrane bound hemoglobin (MBH), membrane lipid-peroxidation (LPO), enzymatic activities of GPx and CAT and the amounts of membrane bound Prx2, GPx and CAT, as well as, clinical and analytical parameters for characterization of HS. We found that unspl HS patients showed clear signs of anemia and in spl HS, a correction to this anemia was observed; the latter patients presented higher levels of OS biomarkers, namely, MBH and LPO. CAT was detected in the membrane of all individuals (control and HS groups), while GPx and Prx2 were only present in HS patients; moreover, their linkage to the membrane (in HS) appears to be cumulative since membrane bound peroxidases amount was higher as the number of peroxidases detected increased. MBH increased with the number/amount of membrane bound peroxidases, however LPO levels remained similar. In conclusion, our data suggest that the binding of these typically cytosolic peroxidases to erythrocyte membrane may be part of a mechanism of membrane protection to maintain its integrity by possibly regulating LPO.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRocha S, Rocha-Pereira P, Cleto E, Ferreira F, Belo L, Santos-Silva A. Linkage of typically cytosolic peroxidases to erythrocyte membrane - A possible mechanism of protection in Hereditary Spherocytosis. Biochim Biophys Acta Biomembr. 2020 Mar 1;1862(3):183172. doi: 10.1016/j.bbamem.2019.183172. Epub 2019 Dec 21. PMID: 31870655.pt_PT
dc.identifier.doi10.1016/j.bbamem.2019.183172pt_PT
dc.identifier.issn1879-2642
dc.identifier.urihttp://hdl.handle.net/10400.16/2488
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0005273619303207?via%3Dihubpt_PT
dc.subjectCatalasept_PT
dc.subjectGlutathione peroxidasept_PT
dc.subjectLipid-peroxidationpt_PT
dc.subjectMembrane oxidative stresspt_PT
dc.subjectMembrane-bound hemoglobinpt_PT
dc.subjectPeroxiredoxin 2pt_PT
dc.titleLinkage of typically cytosolic peroxidases to erythrocyte membrane - A possible mechanism of protection in Hereditary Spherocytosispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceNetherlandspt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage183172pt_PT
oaire.citation.titleBiochimica et biophysica acta. Biomembranespt_PT
oaire.citation.volume1862pt_PT
person.familyNameCleto
person.givenNameMaria Esmeralda da Silva
person.identifier.orcid0000-0001-5416-0120
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication719499e7-2dd9-42f1-86f0-3f9503a683d3
relation.isAuthorOfPublication.latestForDiscovery719499e7-2dd9-42f1-86f0-3f9503a683d3

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