Moura, J.Gonçalves, M:Fonseca, S.Santos, A.Queirós, M.Teixeira, M.Lima, M.2010-07-212010-07-212009http://hdl.handle.net/10400.16/326The complexity of T and NK-cell responses needs a great deal of control in the way these cells respond to stimulation. This is made by a precise regulation of gene expression leading to specific changes of membrane proteins, in order to acquire their full cytotoxic or immunological mediators secreting potential. The immunophenotypic changes occurring in-vivo on blood T and NK-cells in patients with acute and chronic infections have been previously characterized in detail, defining early and late activation related phenotypes, but in-vitro experiments are needed to define the earliest activation stages.engconference object