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- The Anatomic Pathology laboratory adjustments in the era of COVID-19 pandemic: The experience of a laboratory in a Portuguese central hospitalPublication . Lamas, Nuno Jorge; Esteves, Sara; Alves, Joana Raposo; Costa, Francisca Emanuel; Tente, David; Fonseca, Paula; Vizcaíno, JoséThe coronavirus disease 2019 (COVID-19) pandemic has dramatically changed the world over the past weeks, with already 8,25 million infections and 445,000 deaths worldwide, leading to an unprecedented international global effort to contain the virus and prevent its spread. The emergence of novel respiratory viruses such as the SARS-CoV-2 creates dramatic challenges to the healthcare services, including surgical pathology laboratories, despite their extensive daily experience in dealing with biological and chemical hazards. Here, we cover important aspects on the knowledge on COVID-19 gathered during the first six months of the pandemic and address relevant issues on human biological sample handling in the Anatomic Pathology laboratory in the context of COVID-19 global threat. In addition, we detail our strategy to minimize the risk of contamination upon exposure to the different biological products received in the laboratory, which can be of general interest to other laboratories worldwide. Our approach has enabled a safe work environment for laboratory staff, while ensuring the maintenance of high quality standards of the work performed. In times of uncertainty and given the lack of specific guidelines directed at Anatomic Pathology services to better deal with the global COVID-19 public-health emergency, it is essential to share with the community rigorous methodologies that will enable us to better cope with probable novel waves of COVID-19 infection and other viruses that will possibly arise in the near future.
- Compreensão Emocional da Morte Pelas Crianças em Idade Pré-escolar: Uma Dimensão EsquecidaPublication . Silva, Filipa Martins; Lopes, Ana Filipa; Carneiro, Vânia; Campelo, ÁlvaroIntrodução: É ainda parca a evidência científica quanto ao entendimento que as crianças têm sobre o conceito de morte, recentemente apontado pela International Children’s Palliative Care Network como área prioritária de investigação. Em particular, a evicção da emoção nesta área de investigação é uma lacuna importante. Este estudo visa desenvolver uma visão aprofundada da dimensão emocional da compreensão da morte pela criança, procurando, também, relacioná-la com a dimensão cognitiva. Material e Métodos: Entrevistámos crianças (três a seis anos), com recurso a um livro ilustrando o cenário hipotético em que uma criança se deparava com a de morte de um familiar. Colocámos questões para avaliar os subconceitos cognitivos da morte e a dimensão emocional (o que sentiria a criança e o que lhe devia ser dito pelos pais). Resultados: Dos 54 participantes, a maioria disse que a criança se sentiria triste (n = 46, 85%) e que os pais deveriam informá-la (n = 47, 87%); estas respostas não variaram significativamente com a idade. A compreensão cognitiva do conceito de morte das crianças que referiram a tristeza foi significativamente superior. Discussão: Mesmo as crianças mais pequenas sentem a morte, não sendo possível desligar as compreensões cognitiva e emocional. Adicionalmente, as crianças devem ser informadas, com vista a uma elaboração adequada e multidimensional da morte. Conclusão: Este estudo fornece informação valiosa aos profissionais de saúde e outros adultos interessados sobre a forma como a criança em idade pré-escolar se posiciona em relação à morte.
- Impact of the COVID-19 Pandemic on Ophthalmology Residency Training in PortugalPublication . Silva, Nisa; Laiginhas, Rita; Meireles, Angelina; Barbosa Breda, JoãoIntroduction: The purpose of this study was to provide objective and real-life data concerning the impact of the COVID-19 pandemic on ophthalmology residency training in Portugal. Material and Methods: Descriptive survey-based study applied to current Portuguese ophthalmology residents (n = 80 eligible). Results: Seventy-five residents participated (94% response rate). All except one (99%) admitted a change in their routine clinical practice during the pandemic, and most (89%) continued to be engaged in ophthalmology department duties. Twenty-five percent were deployed to COVID-19 units, which was combined with ophthalmological activities in about half of them (47%). A significant proportion of participants stated that they were enrolled in the following ophthalmological activities: emergency/inpatient care (87%), outpatient visits (73%; general 70% vs subspecialty 29%), and surgical procedures (64%). Twenty-five percent did not assist in any outpatient visits and 36% did not participate in any surgical procedures. On a scale from 1 (no impact) to 5 (maximum impact), most participants classified their perceived negative impact of the pandemic on the training program as 3 (24%), 4 (40%), or 5 (27%). Participants highly agreed with the extension of the residency program (80%) in order to to make up for training disruption. Discussion: Most trainees provided ophthalmological care during the pandemic. However, those clinical activities were essentially related to general and emergency care. Surgical experience was significantly curtailed. As such, strategies are needed to guarantee a high-quality learning process. Further studies are required to develop an international perspective on how ophthalmology training programs have been affected so that educational organizations can make recommendations regarding standardized adjustments to training programs. Conclusion: The COVID-19 pandemic has significantly impacted the training of ophthalmology residents nationwide.
- Oral ulcer as presentation of cavitating pulmonary tuberculosisPublication . Fragoso, Joana; Oliveira, Mariana Marques; Gonçalves, Celina; Méndez, Josefina; Sarmento-Castro, RuiTuberculosis is an indolent infection that can invade any organ. Although the most frequent form of presentation is pulmonary, it can have an extra-pulmonary presentation, including rare cases of oral tuberculosis. We present a clinical case of a 44 year-old man, active smoker, with an ulcerated lesion on the posterior third of the tongue, initially interpreted as a probable neoplasm. The pathological study of the biopsy performed on the lesion, showed alterations compatible with a chronic granulomatous process and the presence of acid-fast bacilli. The concomitant diagnosis of pulmonary tuberculosis was made in a subsequent study. The patient started therapy with isoniazid, rifampin, pyrazinamide and ethambutol with complete resolution of the oral lesion and pulmonary tuberculosis. This case exemplifies the importance of including tuberculosis in the differential diagnosis of ulcerated and neoformative lesions and the value of performing a microbiological study alongside the pathological one.
- A systematic review on the effects of endometriosis on sexuality and couple's relationshipPublication . Norinho, P.; Martins, M.; Ferreira, HelderBackground: Endometriosis is likely to affect sexuality and intimate relationships but the effect endometriosis has on partners remains overlooked and the existing studies show conflicting results. The effect of the disease and its treatment on the couple may be pronounced given the absence of an obvious cause or cure, the likelihood of chronic recurring symptoms, and the potential impact on both sex and fertility. Materials and methods: We followed the PRISMA guidelines to conduct this systematic review, which involved a database search of published available research related to the effects of endometriosis treatment on sexual function, couple's relationship and on the partner published between 2000 and 2020. Results: The studies considered revealed that women with endometriosis report a significant effect of the disease on sexuality and relationship. Also, most of the published studies suggest that the impact on partners may be profound, affecting many life domains including sex, intimacy and the relationship in general. Conclusions: Data suggests that male partners should not be overlooked in the treatment of endometriosis and that psychosocial support including sexual and couple therapy might be beneficial.
- Bowel anastomosis leakage following endometriosis surgery: an evidence based analysis of risk factors and prevention techniquesPublication . Vigueras Smith, A; Sumak, R.; Cabrera, R.; Ferreira, HelderBackground: Deep endometriosis most commonly involves the rectosigmoid junction and its management often requires a colorectal resection. Anastomotic leakage is a severe complication after resection and affects 1-6% of the cases. Objective: To evaluate the risk factors related to anastomotic leakage following endometriosis sur-gery, its prevention techniques and the role of protective stomas. Methods: A comprehensive literature review was carried out for English-language publications in Pubmed and Google Scholar. We included all studies including the following MeSH terms and key words: Anastomotic leakage AND bowel surgery OR Endometriosis OR Colorectal surgery OR Bowel endometriosis. Two authors independently made a selection and analysed relevant abstracts according to the aim of this review. Results: Risk factors and preventive measures were categorised considering the patient condition, the intra- operative setting and the surgical procedure itself. Level I and II recommendations include modifiable risk factors such as the use of stapled or handsewn anastomosis; intra-operative air leak test to check the integrity of the anastomosis; systematic use of pelvic and trans-anal drainage; application of protective or ghost ileostomy in low rectal resections; vaginal closure before the bowel resection; use of oral antibiotics the day before surgery and performing partial mesorectal resection near the bowel wall. Diverting stomas may decrease the morbidity and the clinical consequences of leakage over 65% of low rectal resections but may cause significant adverse effects. Conclusion: Evidence-based protective actions are crucial to reduce clinical consequences of anastomotic leakage and to minimise the use of protective stomas in endometriosis surgery.
- β-Adrenoceptor Activation in Breast MCF-10A Cells Induces a Pattern of Catecholamine Production Similar to that of Tumorigenic MCF-7 CellsPublication . Amaro, Filipa; Silva, Dany; Reguengo, Henrique; Oliveira, José Carlos; Quintas, Clara; Vale, Nuno; Gonçalves, Jorge; Fresco, PaulaAdrenaline, which participates in the neuroendocrine response that occurs during stress and perimenopause, may be tumorigenic. This exploratory study aimed at investigating whether non-tumorigenic and tumorigenic human breast epithelial cell lines are able to synthesize adrenaline. The study was carried out in non-tumorigenic (MCF-10A) and tumorigenic (MCF-7) human breast cell lines. Expression of enzymes involved in adrenaline synthesis was characterized by RT-qPCR, immunocytochemistry and western blot. Catecholamines and analogue compounds were quantified by HPLC-ECD. Functional assessment of the impact of drugs on cells' tumorigenic potential was assessed by determination of cell viability and clonogenic ability. Both MCF-10A and MCF-7 cells produce catecholamines, but the capacity to produce adrenaline is lower in MCF-10A cells. β-adrenoceptor activation increases the capacity of MCF-10A cells to produce adrenaline and favor both cell viability and colony formation. It is concluded that exposure of human breast epithelial cells to β-adrenoceptor agonists increases cell proliferation and the capacity to produce adrenaline, creating an autocrine potential to spread these adrenergic effects in a feed-forward loop. It is conceivable that these effects are related to tumorigenesis, bringing a new perspective to understand the claimed anticancer effects of propranolol and the increase in breast cancer incidence caused by stress or during perimenopause.