Repository logo
 
Publication

Circulating endothelial cells in patients with venous thromboembolism and myeloproliferative neoplasms

2013-12Journal article Open access
Simple item page
dc.contributor.authorTorres, C.
dc.contributor.authorFonseca, A.
dc.contributor.authorLeander, M.
dc.contributor.authorMatos, R.
dc.contributor.authorMorais, S.
dc.contributor.authorCampos, M.
dc.contributor.authorLima, M.
dc.date.accessioned2014-10-23T10:51:59Z
dc.date.available2014-10-23T10:51:59Z
dc.date.issued2013-12
dc.description.abstractBackground Circulating endothelial cells (CEC) may be a biomarker of vascular injury and pro-thrombotic tendency, while circulating endothelial progenitor cells (CEP) may be an indicator for angiogenesis and vascular remodelling. However, there is not a universally accepted standardized protocol to identify and quantify these cells and its clinical relevancy remains to be established. Objectives To quantify CEC and CEP in patients with venous thromboembolism (VTE) and with myeloproliferative neoplasms (MPN), to characterize the CEC for the expression of activation (CD54, CD62E) and procoagulant (CD142) markers and to investigate whether they correlate with other clinical and laboratory data. Patients and Methods Sixteen patients with VTE, 17 patients with MPN and 20 healthy individuals were studied. The CEC and CEP were quantified and characterized in the blood using flow cytometry, and the demographic, clinical and laboratory data were obtained from hospital records. Results We found the CEC counts were higher in both patient groups as compared to controls, whereas increased numbers of CEP were found only in patients with MPN. In addition, all disease groups had higher numbers of CD62E+ CEC as compared to controls, whereas only patients with VTE had increased numbers of CD142+ and CD54+ CEC. Moreover, the numbers of total and CD62+ CEC correlated positively with the white blood cells (WBC) counts in both groups of patients, while the numbers of CEP correlated positively with the WBC counts only in patients with MPN. In addition, in patients with VTE a positive correlation was found between the numbers of CD54+ CEC and the antithrombin levels, as well as between the CD142+ CEC counts and the number of thrombotic events. Conclusions Our study suggests that CEC counts may reveal endothelial injury in patients with VTE and MPN and that CEC may express different activation-related phenotypes depending on the disease status.por
dc.description.sponsorshipFunding: Funding source: Unidade Multidisciplinar de Investigação Biomédica, Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto(UMIB/ICBAS/UP (http://www1.icbas.up.pt/umib/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.por
dc.identifier.citationTorres C, Fonseca AM, Leander M, Matos R, Morais S, et al. (2013) Circulating Endothelial Cells in Patients with Venous Thromboembolism and Myeloproliferative Neoplasms. PLoS ONE 8(12): e81574. doi:10.1371/journal.pone.0081574por
dc.identifier.doi10.1371/journal.pone.0081574
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10400.16/1718
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherPublic Library of Sciencepor
dc.relation.publisherversionhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0081574por
dc.titleCirculating endothelial cells in patients with venous thromboembolism and myeloproliferative neoplasmspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceUnited States of Americapor
oaire.citation.titlePLoS Onepor
oaire.citation.volume8(12)por
rcaap.rightsopenAccesspor
rcaap.typearticlepor

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Circulating Endothelial Cells in Patients with Venous.pdf
Size:
890.53 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.35 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

SHC - Artigos publicados em revistas indexadas na Pubmed/Medline
SS - Artigos publicados em revistas indexadas na Medline