Publication
Chemokine Receptor Expression on Normal Blood CD56(+) NK-Cells Elucidates Cell Partners That Comigrate during the Innate and Adaptive Immune Responses and Identifies a Transitional NK-Cell Population
| dc.contributor.author | Lima, M. | |
| dc.contributor.author | Leander, M. | |
| dc.contributor.author | Santos, M. | |
| dc.contributor.author | Santos, A. | |
| dc.contributor.author | Lau, C. | |
| dc.contributor.author | Queirós, M. | |
| dc.contributor.author | Gonçalves, M. | |
| dc.contributor.author | Fonseca, S. | |
| dc.contributor.author | Moura, J. | |
| dc.contributor.author | Teixeira, M. | |
| dc.contributor.author | Orfao, A. | |
| dc.date.accessioned | 2016-07-19T11:51:50Z | |
| dc.date.available | 2016-07-19T11:51:50Z | |
| dc.date.issued | 2015 | |
| dc.description.abstract | Studies of chemokine receptors (CKR) in natural killer- (NK-) cells have already been published, but only a few gave detailed information on its differential expression on blood NK-cell subsets. We report on the expression of the inflammatory and homeostatic CKR on normal blood CD56(+low) CD16(+) and CD56(+high) CD16(-/+low) NK-cells. Conventional CD56(+low) and CD56(+high) NK-cells present in the normal PB do express CKR for inflammatory cytokines, although with different patterns CD56(+low) NK-cells are mainly CXCR1/CXCR2(+) and CXCR3/CCR5(-/+), whereas mostly CD56(+high) NK-cells are CXCR1/CXCR2(-) and CXCR3/CCR5(+). Both NK-cell subsets have variable CXCR4 expression and are CCR4(-) and CCR6(-). The CKR repertoire of the CD56(+low) NK-cells approaches to that of neutrophils, whereas the CKR repertoire of the CD56(+high) NK-cells mimics that of Th1(+) T cells, suggesting that these cells are prepared to migrate into inflamed tissues at different phases of the immune response. In addition, we describe a subpopulation of NK-cells with intermediate levels of CD56 expression, which we named CD56(+int) NK-cells. These NK-cells are CXCR3/CCR5(+), they have intermediate levels of expression of CD16, CD62L, CD94, and CD122, and they are CD57(-) and CD158a(-). In view of their phenotypic features, we hypothesize that they correspond to a transitional stage, between the well-known CD56(+high) and CD56(+low) NK-cells populations. | pt_PT |
| dc.description.sponsorship | This work has been partially supported by a grant of the “Associação Portuguesa Contra a Leucemia” for the project “caracterização imunofenotípica de leucemias e linfomas de células T e NK: padrão de expressão de receptores de quimiocinas como determinantes do tropismo tecidular da célula neoplásica e do comportamento clínico-biológico da doença” and by the “Forum Hematológico do Norte,” Portugal | pt_PT |
| dc.identifier.citation | J Immunol Res. 2015;2015:839684 | pt_PT |
| dc.identifier.doi | 10.1155/2015/839684 | pt_PT |
| dc.identifier.issn | 2314-7156 | |
| dc.identifier.uri | http://hdl.handle.net/10400.16/1961 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Hindawi Publishing Corporation | pt_PT |
| dc.relation.publisherversion | http://www.hindawi.com/journals/jir/2015/839684/ | pt_PT |
| dc.title | Chemokine Receptor Expression on Normal Blood CD56(+) NK-Cells Elucidates Cell Partners That Comigrate during the Innate and Adaptive Immune Responses and Identifies a Transitional NK-Cell Population | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.conferencePlace | Egypt | pt_PT |
| oaire.citation.endPage | 18 | pt_PT |
| oaire.citation.startPage | 839684 | pt_PT |
| oaire.citation.title | Journal of Immunology Research | pt_PT |
| oaire.citation.volume | 2015 | pt_PT |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |