Browsing by Author "Moura, J."
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- Chemokine Receptor Expression on Normal Blood CD56(+) NK-Cells Elucidates Cell Partners That Comigrate during the Innate and Adaptive Immune Responses and Identifies a Transitional NK-Cell PopulationPublication . Lima, M.; Leander, M.; Santos, M.; Santos, A.; Lau, C.; Queirós, M.; Gonçalves, M.; Fonseca, S.; Moura, J.; Teixeira, M.; Orfao, A.Studies of chemokine receptors (CKR) in natural killer- (NK-) cells have already been published, but only a few gave detailed information on its differential expression on blood NK-cell subsets. We report on the expression of the inflammatory and homeostatic CKR on normal blood CD56(+low) CD16(+) and CD56(+high) CD16(-/+low) NK-cells. Conventional CD56(+low) and CD56(+high) NK-cells present in the normal PB do express CKR for inflammatory cytokines, although with different patterns CD56(+low) NK-cells are mainly CXCR1/CXCR2(+) and CXCR3/CCR5(-/+), whereas mostly CD56(+high) NK-cells are CXCR1/CXCR2(-) and CXCR3/CCR5(+). Both NK-cell subsets have variable CXCR4 expression and are CCR4(-) and CCR6(-). The CKR repertoire of the CD56(+low) NK-cells approaches to that of neutrophils, whereas the CKR repertoire of the CD56(+high) NK-cells mimics that of Th1(+) T cells, suggesting that these cells are prepared to migrate into inflamed tissues at different phases of the immune response. In addition, we describe a subpopulation of NK-cells with intermediate levels of CD56 expression, which we named CD56(+int) NK-cells. These NK-cells are CXCR3/CCR5(+), they have intermediate levels of expression of CD16, CD62L, CD94, and CD122, and they are CD57(-) and CD158a(-). In view of their phenotypic features, we hypothesize that they correspond to a transitional stage, between the well-known CD56(+high) and CD56(+low) NK-cells populations.
- CHEMOKINE RECEPTOR REPERTOIRE REFLECTS MATURE T-CELL LYMPHOPROLIFERATIVE DISORDER CLINICAL PRESENTATIONPublication . Moura, J.; Rodrigues, J.; Santos, A.; Teixeira, M.; Queirós, M.; Santos, M.; Gonçalves, M.; Fonseca, S.; Laranjeira, C.; Ribeiro, F.; Acosta, M.; Rodrigues, A.; Júnior, E.; Lima, M.The World Health Organisation classification of mature T-cell lymphoproliferative disorders, combines clinical, morphological and immunophenotypic data. The later majorly contributes for the classification, as well as to the understanding of the malignant T-cell behaviour. The fact that T-cell migration is regulated by chemokines should, in theory, enable us to identify tissue tropism and organ involvement by neoplastic Tcells, through monitoring of chemokine receptor surface expression.
- HUMAN BLOOD T AND NK CELL IN VITRO STIMULATION REVEALS A SIMILAR ACTIVATION PHENOTYPE THAT RESEMBLES EARLY ACTIVATION STAGES DURING ACUTE VIRUS INFECTIONPublication . Moura, J.; Gonçalves, M:; Fonseca, S.; Santos, A.; Queirós, M.; Teixeira, M.; Lima, M.The complexity of T and NK-cell responses needs a great deal of control in the way these cells respond to stimulation. This is made by a precise regulation of gene expression leading to specific changes of membrane proteins, in order to acquire their full cytotoxic or immunological mediators secreting potential. The immunophenotypic changes occurring in-vivo on blood T and NK-cells in patients with acute and chronic infections have been previously characterized in detail, defining early and late activation related phenotypes, but in-vitro experiments are needed to define the earliest activation stages.