Browsing by Author "TEIXEIRA, M.A."
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- Association of CD4+/CD56+/CD57+/CD8+(dim) large granular lymphocytic leukemia, splenic B‐cell lymphoma with circulating villous lymphocytes, and idiopathic erythrocytosisPublication . LIMA, M.; GONCALVES, C.; MARQUES, L.; MARTIN, M.C.; TEIXEIRA, M.A.; QUEIROS, M.L.; SANTOS, A.H.; BALANZATEGUI, A.; GARCIA‐SANZ, R.; PINTO‐RIBEIRO, A.C.; JUSTICA, B.; ORFAO, A.Ann Hematol. 2001 Nov;80(11):685-90. Association of CD4+/CD56+/CD57+/CD8+(dim) large granular lymphocytic leukemia, splenic B-cell lymphoma with circulating villous lymphocytes, and idiopathic erythrocytosis. Lima M, Gonçalves C, Marques L, Martin MC, Teixeira MA, Queirós ML, Santos AH, Balanzategui A, Garcia-Sanz R, Pinto-Ribeiro AC, Justiça B, Orfão A. Service of Clinical Hematology, Hospital Geral Santo António, Porto, Portugal. mmc.lima@clix.pt Abstract In this paper we report a rare association of a splenic marginal zone B-cell lymphoma with villous lymphocytes and a T-cell large granular lymphocytic leukemia coexpressing CD4 and CD8 as well as CD56 and CD57 natural killer-associated markers in an asymptomatic patient investigated because of an occasional finding of erythrocytosis and leukocytosis in routine blood analysis. We also discuss the possible reasons for this particular association. PMID: 11757730 [PubMed - indexed for MEDLINE]
- CD8 (+)/V beta 5.1(+) large granular lymphocyte leukemia associated with autoimmune cytopenias, rheumatoid arthritis and vascular mammary skin lesions: successful response to 2‐deoxycoformycin.Publication . GRANJO, E.; LIMA, M.; CORREIA, T.; LISBOA, C.; MAGALHAES, C; CUNHA, N.; TEIXEIRA, M.A.; QUEIROS, M.L.Hematol Oncol. 2002 Jun;20(2):87-93. Cd8(+)/V beta 5.1(+) large granular lymphocyte leukemia associated with autoimmune cytopenias, rheumatoid arthritis and vascular mammary skin lesions: successful response to 2-deoxycoformycin. Granjo E, Lima M, Correia T, Lisboa C, Magalhães C, Cunha N, Teixeira MA, Queirós ML, Candeias J, Matutes E. Department of Clinical Haematology, Hospital Geral de São João, Porto, Portugal. npp46740@mail.telepac.pt Abstract We report a case of CD8(+)/V beta 5.1(+) T-cell large granular lymphocyte leukemia (T-LGL leukemia) presenting with mild lymphocytosis, severe autoimmune neutropenia, thrombocytopenia, polyarthritis and recurrent infections with a chronic disease course. Immunophenotyping showed an expansion of CD3(+)/TCR alpha beta(+)/CD8(+bright)/CD11c(+)/CD57(-)/CD56(-) large granular lymphocytes with expression of the TCR-V beta 5.1 family. Southern blot analysis revealed a clonal rearrangement of the TCR beta-chain gene. Hematopoietic growth factors, high dose intravenous immunoglobulin and corticosteroids were of limited therapeutic benefit to correct the cytopenias. During the disease course, the patient developed a severe cutaneous leg ulcer and bilateral vascular mammary skin lesions. Treatment with 2-deoxycoformycin resulted in both clinical and hematological complete responses, including the resolution of vascular skin lesions. Combined immuno-staining with relevant T-cell associated and anti-TCR-V beta monoclonal antibodies proved to be a sensitive method to assess the therapeutic effect of 2-deoxycoformicin and to evaluate the residual disease. Copyright 2002 John Wiley & Sons, Ltd. PMID: 12111871 [PubMed - indexed for MEDLINE
- Immunophenotypic characterization of normal blood CD56+lo versus CD56+hi NK‐cell subsets and its impact on the understanding of their tissue distribution and functional propertiesPublication . LIMA, M.; TEIXEIRA, M.A.; QUEIROS, M.L.; LEITE, M.; SANTOS, A.H.; JUSTICA, B.; ORFAO, A.Blood Cells Mol Dis. 2001 Jul-Aug;27(4):731-43. Immunophenotypic characterization of normal blood CD56+lo versus CD56+hi NK-cell subsets and its impact on the understanding of their tissue distribution and functional properties. Lima M, Teixeira MA, Queirós ML, Leite M, Santos AH, Justiça B, Orfão A. Service of Clinical Hematology, Unit of Cytometry, Hospital Geral de Santo António, Porto, Portugal. mmc.lima@clix.pt Abstract In the present study we have compared the immunophenotypic characteristics of the CD56+lo and CD56+hi NK-cell subsets in a group of normal healthy adults. Our results show that CD56+hi NK-cells display greater light-scatter properties than CD56+lo NK-cells at the same time they have higher levels of CD25 and CD122 IL-2 chains, together with a higher reactivity for HLA-DR and CD45RO and lower levels of CD45RA, supporting that, as opposed to the majority of the CD56+lo population, CD56+hi NK-cells might correspond to a subset of activated circulating NK-lymphocytes. Higher expression of the CD2 and CD7 costimulatory molecules found for the CD56+hi NK-cells would support their greater ability to respond to various stimuli. In addition, CD56+hi NK-cells expressed higher levels of several adhesion molecules such as CD2, CD11c, CD44, CD56, and CD62L compared to CD56+lo NK-cells, supporting a particular ability of these cells to migrate from blood to tissues and/or a potential advantage to form conjugates with target cells. Interestingly, CD56+lo and CD56+hi NK-cells showed a different pattern of expression of killer receptors that might determine different activation requirements for each of these NK-cell subsets. For instance, absence or low levels of CD16 expression might explain the lower antibody-dependent cytotoxicity activity of CD56+hi NK-cells. On the other hand, the virtual absence of expression of the CD158a and NKB1 immunoglobulin-like and the greater reactivity for the CD94 lectin-like killer receptors on CD56+hi in comparison to CD56+lo NK-cells might determine different MHC-class I specificities for both NK-cell subsets, a possibility that deserves further studies to be confirmed. PMID: 11778657 [PubMed - indexed for MEDLINE]
- MENÇÕES SOBRE O ESTADO NUTRICIONAL. Nos Registos Clínicos de Doentes HospitalizadosvoPublication . MATOS, L.; TEIXEIRA, M.A.; HENRIQUES, A.; TAVARES, M.M.; ÁLVARES, L.; ANTUNES, A.; AMARAL, T.Está descrito que a frequência de desnutrição associada à doença (DAD) afecta cerca de 30 a 60% dos doentes no momento da admissão hospitalar e cerca de 10% dos indivíduos na comunidade. A DAD tem vindo a ser associada a graves consequências, como ao maior risco de infecções e de disfunção de órgãos e a um aumento significativo, não só da morbilidade e mortalidade, como da frequência e dos custos com os cuidados de saúde. A falta do reconhecimento e da monitorização dos aspectos relacionados com o estado nutricional, têm sido apontados como factores que contribuem para o aumento da frequência de DAD, durante o internamento hospitalar. Foi objectivo deste estudo avaliar a relevância que é dada a aspectos relacionados com o estado nutricional dos doentes (peso, ingestão alimentar) e saber se os doentes em risco nutricional ou desnutridos serão alvo de maior atenção por parte dos profissionais de saúde. Foi recolhida uma amostra sistemática de seis hospitais portugueses, correspondente a 42-50% do total de camas de cada serviço de internamento. Foram critérios de exclusão a doença crítica, a gravidez, a idade inferior a 18 anos, a incapacidade de aplicação do protocolo de rastreio nutricional e o tempo de internamento inferior a 24h. Recolheram- se dados sócio-demográficos, antropométricos, sobre as menções dos processos clínicos respeitantes ao peso, cuidados alimentares/nutricionais prestados e ingestão alimentar/nutricional dos doentes e aplicou-se uma ferramenta de rastreio nutricional (Nutritional Risk Screening 2002). Em 1152 doentes estudados, a frequência de risco nutricional variou entre os 28,5% e 47,3%, enquanto que a frequência de desnutrição antropométrica oscilou entre 6,3% e 14,9%. Dois em cada três doentes tinha menções acerca de cuidados alimentares/ nutricionais prestados nos processos clínicos, mas apenas um em cada três tinha o seu peso medido e registado. Os doentes desnutridos foram pesados com menor frequência mas a sua alimentação e problemas a ela associados foram monitorizados com maior regularidade. A frequência de DAD, no momento de admissão hospitalar, é muito elevada, enquanto que a de menções relevantes para o estado nutricional é muito escassa. A presente investigação reforça a necessidade de investir na sensibilização dos profissionais de saúde, sobre a importância do rastreio e da prescrição/monitorização da alimentação e do peso dos doentes, na admissão e durante todo o internamento hospitalar.
- Reactive phenotypes after acute and chronic NK‐cell activationPublication . LIMA, M; ALMEIDA, J.; TEIXEIRA, M.A.; SANTOS, A.H.; QUEIROS, M.L.; FONSECA, S.; MOURA, J.; GONCALVES, M.; ORFAO, A.; PINTO RIBEIRO, A.C.J Biol Regul Homeost Agents. 2004 Jul-Dec;18(3-4):331-4. Reactive phenotypes after acute and chronic NK-cell activation. Lima M, Almeida J, Teixeira MA, Santos AH, Queirós ML, Fonseca S, Moura J, Gonçalves M, Orfão A, Pinto Ribeiro AC. Service of Clinical Hematology, Laboratory of Cytometry, Hospital Geral de Santo António, Porto, Portugal. mmc.lima@clix.pt Abstract Several phenotypic changes have been shown to occur after NK-cell stimulation, involving molecules that have been proved to regulate NK-cell migration into tissues and NK-cell activation and proliferation as well as target cell recognition and killing. Here, we review the reactive phenotypes observed in vivo after acute and chronic NK-cell activation. PMID: 15786700 [PubMed - indexed for MEDLINE]