SECMF - Serviço de Estomatologia e Cirurgia Maxilo Facial
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- Acinic Cell Carcinoma of the Nasal Lateral WallPublication . Dionísio, Sílvia; Ventura, Eduardo; Gonçalves, Joana; Nobre, Rafael; Marques, HugoPrimary acinic cell carcinoma arising in the nose is exceptionally rare. In this report, we present a unique case of an acinic cell carcinoma of the nasal lateral wall, and it is only the second such case to be reported. We also engage in a systematic review of all 18 cases of acinic cell carcinoma of the nose reported in the literature in English so far.
- Activation of ERK1/2 MAP kinases in familial amyloidotic polyneuropathyPublication . MONTEIRO, F.A.; SOUSA, M.M.; CARDOSO, I.; Barbas-Amaral, J.; GUIMARAES, A.; SARAIVA, M.J.J Neurochem. 2006 Apr;97(1):151-61. Epub 2006 Mar 3. Activation of ERK1/2 MAP kinases in familial amyloidotic polyneuropathy. Monteiro FA, Sousa MM, Cardoso I, do Amaral JB, Guimarães A, Saraiva MJ. Molecular Neurobiology, Instituto de Biologia Celular e Molecular, ICBAS, University of Porto, and Estomatology, Maxillofacial Surgery, Hospital Geral de Santo António, Portugal. Abstract Familial amyloidotic polyneuropathy (FAP) is a neurodegenerative disorder characterized by the extracellular deposition of transthyretin (TTR), especially in the PNS. Given the invasiveness of nerve biopsy, salivary glands (SG) from FAP patients were used previously in microarray analysis; mitogen-activated protein (MAP) kinase phosphatase 1 (MKP-1) was down-regulated in FAP. Results were validated by RT-PCR and immunohistochemistry both in SG and in nerve biopsies of different stages of disease progression. MKP-3 was also down-regulated in FAP SG biopsies. Given the relationship between MKPs and MAPKs, the latter were investigated. Only extracellular signal-regulated kinases 1/2 (ERK1/2) displayed increased activation in FAP SG and nerves. ERK1/2 kinase (MEK1/2) activation was also up-regulated in FAP nerves. In addition, an FAP transgenic mouse model revealed increased ERK1/2 activation in peripheral nerve affected with TTR deposition when compared to control animals. Cultured rat Schwannoma cell line treatment with TTR aggregates stimulated ERK1/2 activation, which was partially mediated by the receptor for advanced glycation end-products (RAGE). Moreover, caspase-3 activation triggered by TTR aggregates was abrogated by U0126, a MEK1/2 inhibitor, indicating that ERK1/2 activation is essential for TTR aggregates-induced cytotoxicity. Taken together, these data suggest that abnormally sustained activation of ERK in FAP may represent an early signaling cascade leading to neurodegeneration. PMID: 16515552 [PubMed - indexed for MEDLINE]
- Biomarkers in the assessment of therapies for familial amyloidotic polyneuropathyPublication . MACEDO, B.; BATISTA, A.R.; Barbas-Amaral, J.; SARAIVA, M.J.Mol Med. 2007 Nov-Dec;13(11-12):584-91. Biomarkers in the assessment of therapies for familial amyloidotic polyneuropathy. Macedo B, Batista AR, do Amaral JB, Saraiva MJ. SourceMolecular Neurobiology, Instituto de Biologia Molecular e Celular, Porto, Portugal. Abstract The identification of specific biomarkers provides opportunities to develop early diagnostic parameters, monitor disease progression, and test drug efficiency in clinical trials. We previously demonstrated that in familial amyloidotic polyneuropathy (FAP) related to the abnormal extracellular tissue deposition of mutant transthyretin (TTR), inflammatory and apoptotic pathways are triggered in the presymptomatic stages of the disease, when nonfibrillar TTR deposits are present. In the present work, to better define biomarkers for future assessment of prophylactic and therapeutic drugs in the treatment of FAP, we extended the search for oxidative stress and apoptotic biomarkers to clinical samples and animal models presenting nonfibrillar and fibrillar TTR. We found that lipid peroxidation measured by hydroxynonenal, oxidative DNA damage measured by 8-hydroxy-2'-deoxyguanosine, and cellular redox homeostasis measured by glutaredoxin 1 were consistently increased in biopsy specimens from FAP patients and in tissues from transgenic mouse models presenting nonfibrillar TTR deposition. Death-receptor Fas, caspase-8, and Bax were also found to be increased, indicative of the involvement of death receptors in the observed apoptosis process. Removal of TTR deposition by an immunization protocol resulted in significant decreases of the selected markers we describe, corroborating the relationship between TTR deposition, oxidative stress, and apoptosis. Taken together, our results provide a robust biomarker profile for initial experimental animal studies and clinical trials to assess the application of the selected markers in therapies aimed at removal and/or inhibition of TTR polymerization. PMID: 17932549 [PubMed - indexed for MEDLINE] PMCID: PMC2017105Free PMC Article Images from this publication.See all images (5) Free text Figure 1 (A) Top panels: Representative 4-hydroxy-2-nonenal (HNE) staining of salivary glands from healthy individuals (left, n = 6) and FAP patients (right, n = 6). Middle panels: Representative HNE staining in stomach tissue of hTTR Met30 mice without TTR deposition (−/−, left, n = 5) and with TTR deposition (+/−, right, n = 5) ... Biomarkers in the Assessment of Therapies for Familial Amyloidotic Polyneuropathy Mol Med. ;13(11-12):584-591.Figure 2 Top 2 panels: Representative Grx1 and Trx1 staining of salivary glands from healthy individuals (left, n = 6) and FAP patients (right, n = 6); nuclear staining is indicated by the arrow. Middle panel: Representative Grx1 staining in stomach tissue of hTTR Met30/(+/−)hsf mice without TTR deposition (−/−, left, n = 5) and wi... Biomarkers in the Assessment of Therapies for Familial Amyloidotic Polyneuropathy Mol Med. ;13(11-12):584-591.Figure 3 Representative FasL, Fas, active caspase-8, active caspase-3, and Bax staining of salivary glands from healthy individuals (left, n = 6) and FAP patients (right, n = 6). 20× magnification. Quantitation of IHC images of FasL, Fas, active caspase-8, active caspase-3, and Bax staining are represented a... Biomarkers in the Assessment of Therapies for Familial Amyloidotic Polyneuropathy Mol Med. ;13(11-12):584-591.Figure 4 Representative Fas (upper panels) and Bax (bottom panels) staining in stomach and DRG tissue of TTR transgenic mice. Upper panels: Stomach tissue of hTTR Met30 mice without TTR deposition (−/−, left, n = 5) and with TTR deposition (+/−, right, n = 5), of hTTR Met30/(+/−)HSF1-KO, without TTR deposition (−/−, left, n = 6) and with TTR de... Biomarkers in the Assessment of Therapies for Familial Amyloidotic Polyneuropathy Mol Med. ;13(11-12):584-591.Figure 5 Representative immunohistochemistry of TTR, BiP, Fas, Bax, 8-OHdG and Grx1 in stomach tissue of TTR Y78F immunized transgenic mice 9–10 months old (right; n = 6) and nonimmunized age-matched controls (left; n = 6). 20× magnification; Quantification of IHC images of TTR, BiP, Fas, Bax, 8-OHdG, and Gr... Biomarkers in the Assessment of Therapies for Familial Amyloidotic Polyneuropathy Mol Med. ;13(11-12):584-591.Publication Types, MeSH Terms, SubstancesPublication Types Research Support, Non-U.S. Gov't MeSH Terms Amyloidosis, Familial/metabolism Amyloidosis, Familial/therapy* Animals Animals, Genetically Modified Apoptosis Biological Markers/metabolism* DNA Damage Immunohistochemistry Mice Oxidative Stress Polyneuropathies/metabolism Polyneuropathies/therapy* Prealbumin/metabolism Substances Biological Markers Prealbumin
- A Case Report of Extreme Oral Lesions: A Rare Indicator of Bullying-Associated Non-suicidal Self-InjuryPublication . Quitério, Anabela; Mendes Abreu, João; Saura, André; Borges, Maria Inês; Corte Real, AnaBullying has reached epidemic proportions, affecting one in three students worldwide. A pervasive issue that carries profound physical, mental, and social consequences, significantly increasing the risk of non-suicidal self-injury (NSSI) and suicidal behaviors among those who experience this type of harassment and hazing. While physicians and most caregivers are fully aware and competent in identifying signs of self-harming behavior such as scratching, cutting, or burning the skin, oral self-injury is often overlooked as a potential indicator and is associated with unintentional soft tissue biting or specific conditions. We present a rare case of a 14-year-old male who sought medical attention due to severe bilateral tongue ulcers, leading to his admittance to the emergency department (ED) with excruciating pain and feeding difficulties. In the reported case, although the traumatic biting of the tongue emerged as the most probable etiological factor, a specific underlying motive and contextual comprehension were initially absent. It was only after successfully establishing a foundation of trust with the patient, enabling an honest response, that it became evident that the observed lesions represented a manifestation of bullying-induced non-suicidal self-injury. However, patients rarely openly acknowledge intentional self-inflicted lesions and/or their experiences of bullying, underscoring the necessity to maintain vigilance for alternative indicators such as behavioral changes or a noticeable decline in academic productivity. The significance of this case also goes beyond its presentation, highlighting the largely unexplored issue of how a patient's dentofacial features can serve as substantial catalysts for bullying. Therefore, it is only through equally prioritizing awareness of uncommon signs, symptoms, and context that one can expedite early diagnosis and intervention, emphasizing the essential need for comprehensive and timely management of such cases.
- Caso estomatológico: Angina de LudwigPublication . Amorim, J.ABSTRACT A 17 year-old-girl was referred to our department because of facial swelling, trismus and pain. She had had previous drainage of the swelling and was on antibioticotherapy. Ludwigs angina was diagnosed; the orthopanthomagraphy showed dental abscess and caries. She had intravenous antibiotherapy and dental extraction with resolution of the condition.
- Caso Estomatológico: Cáries de BiberãoPublication . Amorim, J.
- Caso Estomatológico: Dentes pré-decíduos ou dentes nataisPublication . Amorim, J.
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